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IHC-P analysis of mouse brain tissue using GTX37603 Neurocan antibody.
IHC-P analysis of mouse brain tissue using GTX37603 Neurocan antibody.
IHC-P analysis of mouse brain tissue using GTX37603 Neurocan antibody.

Neurocan antibody

GTX37603
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNCAN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Neurocan antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:400-800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1463
  • Target name
    NCAN
  • Target description
    neurocan
  • Target synonyms
    CSPG3, neurocan core protein, chondroitin sulfate proteoglycan 3 (neurocan), neurocan proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14594
  • Protein Name
    Neurocan core protein
  • Scientific Description
    Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neurocan using anti-Neurocan antibody (A06700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody (Catalog # A06700-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
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