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Neurofibromin antibody

GTX132099
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Neurofibromin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.88 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4763
  • Target name
    NF1
  • Target description
    neurofibromin 1
  • Target synonyms
    NFNS, VRNF, WSS, neurofibromin, neurofibromatosis 1, neurofibromatosis-related protein NF-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21359
  • Protein Name
    Neurofibromin
  • Scientific Description
    This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NF1 using anti-NF1 antibody (A00043-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF1 antigen affinity purified polyclonal antibody (Catalog # A00043-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF1 at approximately 319KD. The expected band size for NF1 is at 319KD.
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