IHC-P analysis of human skeletal muscle tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human spleen tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22796/GTX22796_1124_IHC-P_w_23060620_341.webp "IHC-P analysis of human spleen tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![IHC-P analysis of human tonsil tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22796/GTX22796_1125_IHC-P_w_23060620_571.webp "IHC-P analysis of human tonsil tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![ICC/IF analysis of MCF-7 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22796/GTX22796_462_ICC-IF_w_23060620_567.webp "ICC/IF analysis of MCF-7 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of 293 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22796/GTX22796_463_ICC-IF_w_23060620_608.webp "ICC/IF analysis of 293 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22796/GTX22796_464_ICC-IF_w_23060620_880.webp "ICC/IF analysis of HeLa cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
IHC-P analysis of human skeletal muscle tissue using GTX22796 NFAT2 antibody [7A6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
NFAT2 antibody [7A6]
GTX22796
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHamster, Human, Mouse, Primate, Rabbit, Rat
TargetNFATC1
Overview
- SupplierGeneTex
- Product NameNFAT2 antibody [7A6]
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross react with NFATc2 (NFAT1).
- Application Supplier NoteWB: 1:2,000. ICC/IF: 1:10-1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7A6
- ConjugateUnconjugated
- Gene ID4772
- Target nameNFATC1
- Target descriptionnuclear factor of activated T cells 1
- Target synonymsNFAT transcription complex cytosolic component; NFAT2; NFATc; NF-ATC; NF-ATc1.2; nuclear factor of activated T-cells 'c'; nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
- HostMouse
- IsotypeIgG1
- Protein IDO95644
- Protein NameNuclear factor of activated T-cells, cytoplasmic 1
- Scientific DescriptionThe product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
- ReactivityHamster, Human, Mouse, Primate, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203