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Western Blot analysis of HeLa cells using NFATc3 Polyclonal Antibody
Western Blot analysis of HeLa cells using NFATc3 Polyclonal Antibody
Western Blot analysis of HeLa cells using NFATc3 Polyclonal Antibody

NFATC3 Antibody

CSB-PA003421
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNFATC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFATC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4775
  • Target name
    NFATC3
  • Target description
    nuclear factor of activated T cells 3
  • Target synonyms
    NFAT4; NF-AT4c; NF-ATc3; NFATX; nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12968
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFAT4 using anti-NFAT4 antibody (A02727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4 antigen affinity purified polyclonal antibody (Catalog # A02727-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4 at approximately 115KD. The expected band size for NFAT4 is at 115KD.
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