Western blot of HeLa cell extract.? The membrane was blocked in 5% dry milk for 2 h.? After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.? Washes with buffer were performed 4 times for 5 each.? The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.?? Washes with TBS preceded color development.
Western blot of HeLa cell extract.? The membrane was blocked in 5% dry milk for 2 h.? After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.? Washes with buffer were performed 4 times for 5 each.? The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.?? Washes with TBS preceded color development.
NFkB p105/p50 antibody
GTX27549
ApplicationsGel Shift Assay, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNFKB1
Overview
- SupplierGeneTex
- Product NameNFkB p50 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:5000-1:25000. EMSA: 0.5-1 microL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID4790
- Target nameNFKB1
- Target descriptionnuclear factor kappa B subunit 1
- Target synonymsCVID12; DNA-binding factor KBF1; EBP-1; KBF1; NFkappaB; NF-kappaB; NF-kappa-B1; NF-kappabeta; NF-kB; NF-kB1; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
- HostRabbit
- IsotypeIgG
- Protein IDP19838
- Protein NameNuclear factor NF-kappa-B p105 subunit
- Scientific DescriptionThe transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the disregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimers disease, atherosclerosis and cancer.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus. Adrianto I et al., 2011 Mar, Nat GenetRead more