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WB analysis of Jurkat cells using GTX47774 NFS1 antibody at 0.3125microg/ml. Lane A : marker Lane B : Jurkat cells
WB analysis of Jurkat cells using GTX47774 NFS1 antibody at 0.3125microg/ml. Lane A : marker Lane B : Jurkat cells
WB analysis of Jurkat cells using GTX47774 NFS1 antibody at 0.3125microg/ml. Lane A : marker Lane B : Jurkat cells

NFS1 antibody, Internal

GTX47774
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetNFS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NFS1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9054
  • Target name
    NFS1
  • Target description
    NFS1 cysteine desulfurase
  • Target synonyms
    COXPD52; cysteine desulfurase, mitochondrial; HUSSY-08; IscS; NFS1 nitrogen fixation 1 homolog; NIFS; nitrogen fixation 1 (S. cerevisiae, homolog); nitrogen-fixing bacteria S-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y697
  • Protein Name
    Cysteine desulfurase, mitochondrial
  • Scientific Description
    Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFS1 using anti-NFS1 antibody (A05061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse B16-F10 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFS1 antigen affinity purified polyclonal antibody (Catalog # A05061-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFS1 at approximately 45 kDa. The expected band size for NFS1 is at 50 kDa.
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