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WB analysis of mouse lung tissue lysate using GTX03452 NG2 antibody. The lane on the left was treated with blocking peptide.
WB analysis of mouse lung tissue lysate using GTX03452 NG2 antibody. The lane on the left was treated with blocking peptide.
WB analysis of mouse lung tissue lysate using GTX03452 NG2 antibody. The lane on the left was treated with blocking peptide.

NG2 antibody

GTX03452
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCSPG4
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Overview

  • Supplier
    GeneTex
  • Product Name
    NG2 antibody
  • Delivery Days Customer
    12
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1464
  • Target name
    CSPG4
  • Target description
    chondroitin sulfate proteoglycan 4
  • Target synonyms
    CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated), chondroitin sulfate proteoglycan NG2, melanoma chondroitin sulfate proteoglycan, melanoma-associated chondroitin sulfate proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UVK1
  • Protein Name
    Chondroitin sulfate proteoglycan 4
  • Scientific Description
    A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NG2/CSPG4 using anti-NG2/CSPG4 antibody (A03394-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NG2/CSPG4 antigen affinity purified polyclonal antibody (Catalog # A03394-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NG2/CSPG4 at approximately 310 kDa. The expected band size for NG2/CSPG4 is at 251 kDa.
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