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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NG2 antibody (GTX130401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NG2 antibody (GTX130401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NG2 antibody (GTX130401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NG2 antibody

GTX130401
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCSPG4
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Overview

  • Supplier
    GeneTex
  • Product Name
    NG2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.14 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1464
  • Target name
    CSPG4
  • Target description
    chondroitin sulfate proteoglycan 4
  • Target synonyms
    CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated), chondroitin sulfate proteoglycan NG2, melanoma chondroitin sulfate proteoglycan, melanoma-associated chondroitin sulfate proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UVK1
  • Protein Name
    Chondroitin sulfate proteoglycan 4
  • Scientific Description
    A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NG2/CSPG4 using anti-NG2/CSPG4 antibody (A03394-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NG2/CSPG4 antigen affinity purified polyclonal antibody (Catalog # A03394-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NG2/CSPG4 at approximately 310 kDa. The expected band size for NG2/CSPG4 is at 251 kDa.
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