More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NGF Antibody (clone B64, Preservative Free)

NGF Antibody (clone B64, Preservative Free)

LS-C819104
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetNGF
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NGF Antibody (clone B64, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap) 1:250-1:2000; ELISA(Cap): This antibody will detect Human NGF / NGFB / beta-NGF in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.. ELISA (1:5000 - 1:10000) ELISA(Cap) 1:250-1:2000; ELISA(Cap): This antibody will detect Human NGF / NGFB / beta-NGF in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B64
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4803
  • Target name
    NGF
  • Target description
    nerve growth factor
  • Target synonyms
    Beta-NGF, HSAN5, NGFB, beta-nerve growth factor, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit, pro-nerve growth factor long
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of Jurkat cells using NGF Polyclonal Antibody
Product group Antibodies
Cusabio
NGF AntibodyCSB-PA003446
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNGF
  • SizePrice
Product group Antibodies
GeneTex
NGF beta antibody [N1C3-2]GTX112043
ApplicationsWestern Blot
TargetNGF
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti Human NGF (Nerve Growth Factor)L146M
ApplicationsImmunoPrecipitation, ELISA, Neutralisation/Blocking
TargetNGF
  • SizePrice
Product group Antibodies
MedChem Express
NGF Antibody (YA3533, Rabbit)HY-P81121
TargetNGF
  • SizePrice
Product group Antibodies
BPS Bioscience
Anti-Beta NGF (Nerve Growth Factor) Neutralizing Antibody100436
ApplicationsNeutralisation/Blocking
TargetNGF
  • SizePrice
Figure 1. Western blot analysis of NGF using anti-NGF antibody (A00341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27KD. The expected band size for NGF is at 27KD.
Product group Antibodies
Boster Bio
Anti-NGF Antibody Picoband(r)A00341-1-CARRIER-FREE
ApplicationsWestern Blot
TargetNGF
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NGF Antibody144-00258
ApplicationsWestern Blot
TargetNGF
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-NGF AntibodyA101608
ApplicationsWestern Blot, ELISA
  • SizePrice
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Product group Antibodies
References
Bioss
NGFB Polyclonal AntibodyBS-0067R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNGF
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Nerve growth factor [mab 911]Ab03301-1.1
ApplicationsELISA, Neutralisation/Blocking, Other Application
TargetNGF
  • SizePrice