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IHC-P analysis of colon adenocarcinoma tissue using GTX84039 NIT2 antibody [3D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of colon adenocarcinoma tissue using GTX84039 NIT2 antibody [3D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of colon adenocarcinoma tissue using GTX84039 NIT2 antibody [3D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50

NIT2 antibody [3D5]

GTX84039
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetNIT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NIT2 antibody [3D5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D5
  • Concentration
    0.79 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56954
  • Target name
    NIT2
  • Target description
    nitrilase family member 2
  • Target synonyms
    HEL-S-8a, omega-amidase NIT2, Nit protein 2, epididymis secretory sperm binding protein Li 8a, nitrilase homolog 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9NQR4
  • Protein Name
    Omega-amidase NIT2
  • Scientific Description
    Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NIT2 using anti-NIT2 antibody (A05891-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIT2 antigen affinity purified polyclonal antibody (Catalog # A05891-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NIT2 at approximately 33 kDa. The expected band size for NIT2 is at 33 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA187627(NIT2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
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