![NKG2D antibody [N3C2], Internal detects NKG2D protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NKG2D stained by NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm. NKG2D antibody [N3C2], Internal detects NKG2D protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NKG2D stained by NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104761/GTX104761_44510_20230210_ICC_IF_23021401_259.webp)
NKG2D antibody [N3C2], Internal detects NKG2D protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NKG2D stained by NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.
NKG2D antibody [N3C2], Internal
GTX104761
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKLRK1
Overview
- SupplierGeneTex
- Product NameNKG2D antibody [N3C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.56 mg/ml
- ConjugateUnconjugated
- Gene ID22914
- Target nameKLRK1
- Target descriptionkiller cell lectin like receptor K1
- Target synonymsCD314, D12S2489E, KLR, NKG2-D, NKG2D, NKG2-D type II integral membrane protein, NK cell receptor D, NKG2-D-activating NK receptor, killer cell lectin-like receptor subfamily K, member 1
- HostRabbit
- IsotypeIgG
- Protein IDP26718
- Protein NameNKG2-D type II integral membrane protein
- Scientific DescriptionNatural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![NKG2D antibody [N3C2], Internal detects NKG2D protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. NKG2D antibody [N3C2], Internal (GTX104761) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min NKG2D antibody [N3C2], Internal detects NKG2D protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. NKG2D antibody [N3C2], Internal (GTX104761) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX104761/GTX104761_39666_IHC_w_23060120_997.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NKG2D antibody [N3C2], Internal (GTX104761) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104761/GTX104761_44468_20230616_WB_B_23062019_934.webp)





![IHC-Fr analysis of human spleen tissue using GTX42086 NKG2D antibody [1D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX42086/GTX42086_5046_IHC-Fr_w_23051500_658.webp)