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Western Blot analysis of 3T3 cells using CD314 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD314 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD314 Polyclonal Antibody

KLRK1 Antibody

CSB-PA008277
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKLRK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KLRK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22914
  • Target name
    KLRK1
  • Target description
    killer cell lectin like receptor K1
  • Target synonyms
    CD314, D12S2489E, KLR, NKG2-D, NKG2D, NKG2-D type II integral membrane protein, NK cell receptor D, NKG2-D-activating NK receptor, killer cell lectin-like receptor subfamily K, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26718
  • Protein Name
    NKG2-D type II integral membrane protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NKG2D using anti-NKG2D antibody (A00661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lymph tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKG2D antigen affinity purified polyclonal antibody (Catalog # A00661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKG2D at approximately 35KD. The expected band size for NKG2D is at 25KD.
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