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Western Blot analysis of K562 cells using Cryopyrin Polyclonal Antibody.
Western Blot analysis of K562 cells using Cryopyrin Polyclonal Antibody.
Western Blot analysis of K562 cells using Cryopyrin Polyclonal Antibody.

NLRP3 Antibody

CSB-PA005618
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNLRP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NLRP3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114548
  • Target name
    NLRP3
  • Target description
    NLR family pyrin domain containing 3
  • Target synonyms
    AGTAVPRL; AII; AVP; C1orf7; caterpiller protein 1.1; CIAS1; CLR1.1; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96P20
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLRP3 using anti-NLRP3 antibody (A00034-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (Catalog # A00034-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 118KD. The expected band size for NLRP3 is at 118KD.
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