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NLRX1 Antibody

LS-C333745
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNLRX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NLRX1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 101kDa/107kDa, while the observed MW by Western blot was 108kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB The predicted MW is 101kDa/107kDa, while the observed MW by Western blot was 108kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79671
  • Target name
    NLRX1
  • Target description
    NLR family member X1
  • Target synonyms
    caterpiller protein 11.3; CLR11.3; DLNB26; NLR family member X1; NOD26; NOD5; NOD9; NOD-like receptor X1; nucleotide-binding oligomerization domain protein 26; nucleotide-binding oligomerization domain protein 5; nucleotide-binding oligomerization domain protein 9; nucleotide-binding oligomerization domain, leucine rich repeat containing X1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRX1 antigen affinity purified polyclonal antibody (Catalog # A04980-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRX1 at approximately 108 kDa. The expected band size for NLRX1 is at 108 kDa.
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Lane 1: Hela cell lysates; Lane 2: NLRX1 knockout (KO) HeLa cell lysates probed with NLRX1 Polyclonal Antibody, Unconjugated (bs-55154R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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