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NLRX1 Antibody

CAB4976
Assay Genie
TargetNLRX1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NLRX1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID79671
  • Target name
    NLRX1
  • Target description
    NLR family member X1
  • Target synonyms
    caterpiller protein 11.3; CLR11.3; DLNB26; NLR family member X1; NOD26; NOD5; NOD9; NOD-like receptor X1; nucleotide-binding oligomerization domain protein 26; nucleotide-binding oligomerization domain protein 5; nucleotide-binding oligomerization domain protein 9; nucleotide-binding oligomerization domain, leucine rich repeat containing X1
  • Host
    Rabbit
  • Protein IDQ86UT6
  • Protein Name
    NLR family member X1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRX1 antigen affinity purified polyclonal antibody (Catalog # A04980-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRX1 at approximately 108 kDa. The expected band size for NLRX1 is at 108 kDa.
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