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Western Blot analysis of Mouse spleen and intestinum crassum tissue using NME6 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse spleen and intestinum crassum tissue using NME6 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse spleen and intestinum crassum tissue using NME6 Polyclonal Antibody at dilution of 1:400

NME6 Polyclonal Antibody

E-AB-14250
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NME6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Inhibitor of p53-induced apoptosis.
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of NME6 using anti-NME6 antibody (A10922-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Lncap whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME6 antigen affinity purified polyclonal antibody (Catalog # A10922-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NME6 at approximately 21 kDa. The expected band size for NME6 is at 21 kDa.
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