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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA015942LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA015942LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA015942LA01HU at dilution of 1:100

NOS1AP Antibody

CSB-PA015942LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNOS1AP
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOS1AP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9722
  • Target name
    NOS1AP
  • Target description
    nitric oxide synthase 1 adaptor protein
  • Target synonyms
    6330408P19Rik; CAPON; carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON); C-terminal PDZ ligand of neuronal nitric oxide synthase protein; ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain; nitric oxide synthase 1 (neuronal) adaptor protein; NPHS22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75052
  • Protein Name
    Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
  • Scientific Description
    Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPON/NOS1AP antigen affinity purified polyclonal antibody (Catalog # A03060-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPON/NOS1AP at approximately 60 kDa. The expected band size for CAPON/NOS1AP is at 56 kDa.
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