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NOS1AP / CAPON Antibody

LS-C678837
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNOS1AP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NOS1AP / CAPON Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9722
  • Target name
    NOS1AP
  • Target description
    nitric oxide synthase 1 adaptor protein
  • Target synonyms
    6330408P19Rik, CAPON, NPHS22, carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON), C-terminal PDZ ligand of neuronal nitric oxide synthase protein, ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain, nitric oxide synthase 1 (neuronal) adaptor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPON/NOS1AP antigen affinity purified polyclonal antibody (Catalog # A03060-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPON/NOS1AP at approximately 60 kDa. The expected band size for CAPON/NOS1AP is at 56 kDa.
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