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Western Blot analysis of various samples using NOS3 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NOS3 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NOS3 Polyclonal Antibody at dilution of 1:1000.

NOS3 Polyclonal Antibody

E-AB-70035
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NOS3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human K562 whole cell lysates Lane 3: human HepG2 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidne tissue lysates Lane 7: mouse small intestine tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.
Product group Antibodies
Boster Bio
Anti-eNOS/NOS3 Antibody Picoband(r)A01604-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNOS3
  • SizePrice
Product group Antibodies
Biosynth
eNOS antibody70R-14249
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Product group Antibodies
RayBiotech
Anti-P-eNOS (1172-1174aa) Antibody130-10610
ApplicationsELISA
ReactivityHuman
TargetNOS3
  • SizePrice
Product group Antibodies
Abbexa
eNOS (Ab-615) AntibodyABX249427
ApplicationsWestern Blot
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Cultured endothelial cells labeled with Anti-Nos-3/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.
Product group Antibodies
References
Bioss
eNOS Polyclonal AntibodyBS-0163R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
TargetNOS3
  • SizePrice
Western blot analysis of Rat Heart Tissue, diluted at 1:1000.
Product group Antibodies
Cusabio
NOS3 Monoclonal AntibodyCSB-MA000326
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNOS3
  • SizePrice
Product group Antibodies
Biomatik
Endothelial NOS (eNOS) Polyclonal AntibodyCAU26913
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Porcine
TargetNOS3
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Product group Antibodies
Assay Genie
COQ9 AntibodyCAB15480
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TargetNOS3
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Product group Antibodies
MedChem Express
eNOS Antibody(Rabbit)HY-P80656
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eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
References
GeneTex
eNOS (phospho Ser1177) antibodyGTX129058
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetNOS3
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