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ProductsBack/NOTCH1 Antibody (aa2487-2501)

NOTCH1 Antibody (aa2487-2501)

LS-C16928
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNOTCH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NOTCH1 Antibody (aa2487-2501)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in, ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500.. ELISA (1:10000 - 1:50000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000) Suitable for use in, ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4851
  • Target name
    NOTCH1
  • Target description
    notch receptor 1
  • Target synonyms
    AOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Notch1 using anti-Notch1 antibody (A00033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Notch1 antigen affinity purified polyclonal antibody (Catalog # A00033-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Notch1 at approximately 272KD. The expected band size for Notch1 is at 272KD.
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