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ProductsBack/NR0B1 Antibody - middle region (ARP31632_P050)

NR0B1 Antibody - middle region (ARP31632_P050)

ARP31632_P050
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNR0B1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    NR0B1 Antibody - middle region (ARP31632_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID190
  • Target name
    NR0B1
  • Target description
    nuclear receptor subfamily 0 group B member 1
  • Target synonyms
    AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2, nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, nuclear hormone receptor, nuclear receptor DAX-1
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (A01521-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (Catalog # A01521-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.
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