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Western Blot analysis of HeLa cells using LXRalpha Polyclonal Antibody
Western Blot analysis of HeLa cells using LXRalpha Polyclonal Antibody
Western Blot analysis of HeLa cells using LXRalpha Polyclonal Antibody

NR1H3 Antibody

CSB-PA004906
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNR1H3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR1H3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10062
  • Target name
    NR1H3
  • Target description
    nuclear receptor subfamily 1 group H member 3
  • Target synonyms
    LXR-a, LXRA, RLD-1, oxysterols receptor LXR-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13133
  • Protein Name
    Oxysterols receptor LXR-alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NR1H3 using anti-NR1H3 antibody (A03331-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1H3 antigen affinity purified polyclonal antibody (Catalog # A03331-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1H3 at approximately 50 kDa. The expected band size for NR1H3 is at 50 kDa.
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ReactivityHuman, Mouse, Rat
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