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ProductsBack/NR1H3 / LXR Alpha Antibody

NR1H3 / LXR Alpha Antibody

LS-C768622
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetNR1H3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NR1H3 / LXR Alpha Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:400) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10062
  • Target name
    NR1H3
  • Target description
    nuclear receptor subfamily 1 group H member 3
  • Target synonyms
    LXR-a, LXRA, RLD-1, oxysterols receptor LXR-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NR1H3 using anti-NR1H3 antibody (A03331-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1H3 antigen affinity purified polyclonal antibody (Catalog # A03331-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1H3 at approximately 50 kDa. The expected band size for NR1H3 is at 50 kDa.
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