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Western Blot analysis of COS7 cells using Nur77 Polyclonal Antibody
Western Blot analysis of COS7 cells using Nur77 Polyclonal Antibody
Western Blot analysis of COS7 cells using Nur77 Polyclonal Antibody

NR4A1 Antibody

CSB-PA003493
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetNR4A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR4A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3164
  • Target name
    NR4A1
  • Target description
    nuclear receptor subfamily 4 group A member 1
  • Target synonyms
    GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3, nuclear receptor subfamily 4immunitygroup A member 1, ST-59, TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, nuclear hormone receptor NUR/77, orphan nuclear receptor HMR, orphan nuclear receptor TR3, steroid receptor TR3, testicular receptor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22736
  • Protein Name
    Nuclear receptor subfamily 4 group A member 1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUR77 using anti-NUR77 antibody (PB9766). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUR77 antigen affinity purified polyclonal antibody (Catalog # PB9766) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUR77 at approximately 67 kDa. The expected band size for NUR77 is at 67 kDa.
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