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ProductsBack/NR4A1 / NUR77 Antibody

NR4A1 / NUR77 Antibody

LS-C746761
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetNR4A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NR4A1 / NUR77 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 33kDa/64kDa/65kDa, while the observed MW by Western blot was 71kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 33kDa/64kDa/65kDa, while the observed MW by Western blot was 71kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3164
  • Target name
    NR4A1
  • Target description
    nuclear receptor subfamily 4 group A member 1
  • Target synonyms
    GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3, nuclear receptor subfamily 4immunitygroup A member 1, ST-59, TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, nuclear hormone receptor NUR/77, orphan nuclear receptor HMR, orphan nuclear receptor TR3, steroid receptor TR3, testicular receptor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUR77 using anti-NUR77 antibody (PB9766). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUR77 antigen affinity purified polyclonal antibody (Catalog # PB9766) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUR77 at approximately 67 kDa. The expected band size for NUR77 is at 67 kDa.
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