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Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody
Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody
Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody

NRAS/HRAS/KRAS Antibody

CSB-PA003358
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHRAS
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Overview

  • Supplier
    Cusabio
  • Product Name
    NRAS/HRAS/KRAS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3265
  • Target name
    HRAS
  • Target description
    HRas proto-oncogene, GTPase
  • Target synonyms
    C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras, GTPase HRas, GTP- and GDP-binding peptide B, Ha-Ras1 proto-oncoprotein, Harvey rat sarcoma viral oncogene homolog, Harvey rat sarcoma viral oncoprotein, Ras family small GTP binding protein H-Ras, c-has/bas p21 protein, p19 H-RasIDX protein, transformation gene: oncogene HAMSV, transforming protein p21, v-Ha-ras Harvey rat sarcoma viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01111
  • Protein Name
    GTPase NRas
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GTPase HRAS using anti-GTPase HRAS antibody (A00114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPase HRAS antigen affinity purified polyclonal antibody (Catalog # A00114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPase HRAS at approximately 21 kDa. The expected band size for GTPase HRAS is at 21 kDa.
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