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ProductsBack/V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Polyclonal Antibody

V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Polyclonal Antibody

CAU26195
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetHRAS
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Overview

  • Supplier
    Biomatik
  • Product Name
    V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.27 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3265
  • Target name
    HRAS
  • Target description
    HRas proto-oncogene, GTPase
  • Target synonyms
    C-BAS/HAS; C-HA-RAS1; c-has/bas p21 protein; C-H-RAS; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; HAMSV; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS1; H-RASIDX; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; RASH1; transformation gene: oncogene HAMSV; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog
  • Host
    Rabbit
  • Protein IDP01112
  • Protein Name
    GTPase HRas
  • Scientific Description
    The V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GTPase HRAS using anti-GTPase HRAS antibody (A00114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPase HRAS antigen affinity purified polyclonal antibody (Catalog # A00114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPase HRAS at approximately 21 kDa. The expected band size for GTPase HRAS is at 21 kDa.
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