Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
![NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_42004_20160720_IFA_w_23060119_806.webp "NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_43552_20200117_WB_treatment_MG132_competitor_watermark_w_23060119_423.webp "Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_44111_20201127_IHC-P_w_23060119_749.webp "NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_43677_20210122_ICC_IF_w_23060119_402.webp "NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.")
![NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_44020_20210924_ICC_IF_treatment_MG132_w_23060119_837.webp "NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_43636_20200311_ICC_IF_w_23060119_425.webp "NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.")
![Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103322/GTX103322_43054_20190118_WB_M_treatment_LPS_w_23060119_100.webp "Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
NRF2 antibody [N2C2], Internal
GTX103322
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAvian, Human, Mouse, Plant, Reptile, Rat, Zebra Fish
TargetNFE2L2
Overview
- SupplierGeneTex
- Product NameNRF2 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.21 mg/ml
- ConjugateUnconjugated
- Gene ID4780
- Target nameNFE2L2
- Target descriptionNFE2 like bZIP transcription factor 2
- Target synonymsHEBP1, IMDDHH, NRF2, Nrf-2, nuclear factor erythroid 2-related factor 2, nuclear factor erythroid-derived 2-like 2, nuclear factor, erythroid 2 like 2
- HostRabbit
- IsotypeIgG
- Protein IDQ16236
- Protein NameNuclear factor erythroid 2-related factor 2
- Scientific DescriptionNFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]
- ReactivityAvian, Human, Mouse, Plant, Reptile, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li LX, Wang L, Wang S, et al. Allyl methyl disulfide (AMDS) prevents N,N-dimethyl formamide-induced liver damage by suppressing oxidative stress and NLRP3 inflammasome activation. Food Chem Toxicol. 2023,182:114198. doi: 10.1016/j.fct.2023.114198Read this paper
- Sulistyowati E, Huang SE, Cheng TL, et al. Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress. Int J Mol Sci. 2023,24(21). doi: 10.3390/ijms242116004Read this paper
- Hirasawa I, Odagiri H, Park G, et al. Anti-inflammatory effects of cold atmospheric plasma irradiation on the THP-1 human acute monocytic leukemia cell line. PLoS One. 2023,18(10):e0292267. doi: 10.1371/journal.pone.0292267Read this paper
- Arellano Buendia AS, Juárez Rojas JG, García-Arroyo F, et al. Antioxidant and anti-inflammatory effects of allicin in the kidney of an experimental model of metabolic syndrome. PeerJ. 2023,11:e16132. doi: 10.7717/peerj.16132Read this paper
- Al-Zahaby SA, Farag MR, Alagawany M, et al. Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels. Animals (Basel). 2023,13(18). doi: 10.3390/ani13182867Read this paper
- Sánchez-Gloria JL, Martínez-Olivares CE, Del Valle-Mondragón L, et al. Allicin, an Emerging Treatment for Pulmonary Arterial Hypertension: An Experimental Study. Int J Mol Sci. 2023,24(16). doi: 10.3390/ijms241612959Read this paper
- Abd El-Emam MM, Mostafa M, Farag AA, et al. The Potential Effects of Quercetin-Loaded Nanoliposomes on Amoxicillin/Clavulanate-Induced Hepatic Damage: Targeting the SIRT1/Nrf2/NF-κB Signaling Pathway and Microbiota Modulation. Antioxidants (Basel). 2023,12(8). doi: 10.3390/antiox12081487Read this paper
- Liu S, Zhang Y, Zheng X, et al. Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related Phenotype. Nutrients. 2023,15(9). doi: 10.3390/nu15092117Read this paper
- Richter FC, Friedrich M, Kampschulte N, et al. Adipocyte autophagy limits gut inflammation by controlling oxylipin and IL-10. EMBO J. 2023,42(6):e112202. doi: 10.15252/embj.2022112202Read this paper
- Zhang H, Zeng J, Li J, et al. Sivelestat sodium attenuates acute lung injury by inhibiting JNK/NF-κB and activating Nrf2/HO-1 signaling pathways. Biomol Biomed. 2023,23(3):457-470. doi: 10.17305/bb.2022.8549Read this paper
Datasheet
Related products
Product group Antibodies
Anti-NFE2L2 Antibody144-01244
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetNFE2L2
- SizePrice
Product group Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNFE2L2
- SizePrice
Product group Antibodies
NFE2L2 AntibodyCSB-PA003481
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNFE2L2
- SizePrice
Product group Antibodies
References
Nrf2 Polyclonal AntibodyBS-1074R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNFE2L2
- SizePrice
Product group Antibodies
NFE2L2 / NRF2 AntibodyLS-C746748
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetNFE2L2
- SizePrice
Product group Antibodies
Anti-Nrf2 AntibodyA83079
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
- SizePrice
Product group Antibodies
References
NRF2 antibodyGTX55732
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Insect, Mouse, Rat
TargetNFE2L2
- SizePrice
![Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL3183] (GTX640822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640822/GTX640822_T-45495_20240809_WB_M_treatment_MG132_24081300_525.webp)
Product group Antibodies
NRF2 antibody [HL3183]GTX640822
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetNFE2L2
- SizePrice
Product group Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetNFE2L2
- SizePrice