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ProductsBack/Goat anti-NRF2 (aa445-458)

Goat anti-NRF2 (aa445-458)

EB11042
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetNFE2L2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NRF2 (aa445-458) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4780
  • Target name
    NFE2L2
  • Target description
    nuclear factor, erythroid 2 like 2
  • Target synonyms
    HEBP1; IMDDHH; NRF2; Nrf-2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006155.2; NP_001138884.1; NP_001138885.1; NP_001300831.1;. GeneIDs all Nonhuman: 18024 (mouse); 83619 (rat);. Purification: Antigen affinity purified. Names and symbols: NFE2L2; nuclear factor (erythroid-derived 2)-like 2; NRF2; HEBP1; NF-E2-related factor 2; NFE2-related factor 2; OTTHUMP00000205251; OTTHUMP00000207980; nuclear factor erythroid 2-related factor 2; nuclear factor, erythroid derived 2, like 2
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFE2L2 using anti-NFE2L2 antibody (A00078-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2L2 antigen affinity purified polyclonal antibody (Catalog # A00078-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2L2 at approximately 100KD. The expected band size for NFE2L2 is at 100KD.
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