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Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining

Nrf2 Polyclonal Antibody

BS-1074R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNFE2L2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Nrf2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4780
  • Target name
    NFE2L2
  • Target description
    nuclear factor, erythroid 2 like 2
  • Target synonyms
    HEBP1; IMDDHH; NRF2; Nrf-2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16236
  • Protein Name
    Nuclear factor erythroid 2-related factor 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Neuroprotective effects of maize tetrapeptide-anchored gold nanoparticles in Alzheimers disease. Zhang J et al., 2021 Apr, Colloids Surf B Biointerfaces
    Read more
  • Selenium Yeast Alleviates Ochratoxin A-Induced Hepatotoxicity via Modulation of the PI3K/AKT and Nrf2/Keap1 Signaling Pathways in Chickens. Li P et al., 2020 Feb 25, Toxins (Basel)
    Read more
  • A novel acidic polysaccharide from the residue of Panax notoginseng and its hepatoprotective effect on alcoholic liver damage in mice. Wang C et al., 2020 Apr 15, Int J Biol Macromol
    Read more
  • Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Han C et al., 2020 Mar 15, Ecotoxicol Environ Saf
    Read more
  • Effects of Essential Trace Elements and Oxidative Stress on Endemic Arsenism Caused by Coal Burning in PR China. Hu Y et al., 2020 Nov, Biol Trace Elem Res
    Read more
  • Imidacloprid-induced liver fibrosis in quails via activation of the TGF-beta1/Smad pathway. Lv Y et al., 2020 Feb 25, Sci Total Environ
    Read more
  • Effect of Illicium verum or Eucommia ulmoides leaf extracts on the anti-stress ability, and mRNA and protein expression of Nrf2 and TNF-alpha in Duroc x Landrace x Yorkshire and Chinese native Licha-black nursery piglets. Jiang S et al., 2020 Jul, J Anim Physiol Anim Nutr (Berl)
    Read more
  • Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Li J et al., 2019 Oct 21, Vet Res
    Read more
  • Endogenous ET-1 promotes ANP secretion through activation of COX2-L-PGDS-PPARgamma signaling in hypoxic beating rat atria. Li X et al., 2019 Dec, Peptides
    Read more
  • Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Ma Q et al., 2019, Am J Transl Res
    Read more

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Figure 1. Western blot analysis of NFE2L2 using anti-NFE2L2 antibody (A00078-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2L2 antigen affinity purified polyclonal antibody (Catalog # A00078-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2L2 at approximately 100KD. The expected band size for NFE2L2 is at 100KD.
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