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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: CSB-PA793948(NRGN Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: CSB-PA793948(NRGN Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: CSB-PA793948(NRGN Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

NRGN Antibody

CSB-PA793948
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNRGN
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Overview

  • Supplier
    Cusabio
  • Product Name
    NRGN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4900
  • Target name
    NRGN
  • Target description
    neurogranin
  • Target synonyms
    calmodulin-binding protein; hng; neurogranin; neurogranin (protein kinase C substrate, RC3); protein kinase C substrate; RC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92686
  • Protein Name
    Neurogranin
  • Scientific Description
    Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neurogranin/NRGN using anti-Neurogranin/NRGN antibody (A05781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurogranin/NRGN antigen affinity purified polyclonal antibody (Catalog # A05781-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurogranin/NRGN at approximately 11 kDa. The expected band size for Neurogranin/NRGN is at 8 kDa.
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Positive sample：Paraformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG/Neurogranin) at 37°C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4°C, followed by secondary antibody and DAB staining.
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