FACS analysis of HEK293T cells transfected with either NSE2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83996 NSE2 antibody [4H9].
![WB analysis of various cell lines using GTX83996 NSE2 antibody [4H9]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83996/GTX83996_3802_WB_w_23061420_883.webp "WB analysis of various cell lines using GTX83996 NSE2 antibody [4H9]. Loading : 35 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with NSE2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83996 NSE2 antibody [4H9]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83996/GTX83996_4054_WB_w_23061420_874.webp "WB analysis of HEK293T cells transfected with NSE2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83996 NSE2 antibody [4H9]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83996 NSE2 antibody [4H9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83996/GTX83996_905_ICCIF_w_23061420_733.webp "ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83996 NSE2 antibody [4H9].")
FACS analysis of HEK293T cells transfected with either NSE2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83996 NSE2 antibody [4H9].
NSE2 antibody [4H9]
GTX83996
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNSMCE2
Overview
- SupplierGeneTex
- Product NameNSE2 antibody [4H9] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4H9
- Concentration0.81 mg/ml
- ConjugateUnconjugated
- Gene ID286053
- Target nameNSMCE2
- Target descriptionNSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
- Target synonymsC8orf36; E3 SUMO-protein ligase NSE2; E3 SUMO-protein transferase NSE2; methyl methanesulfonate sensitivity gene 21; MMS21; non-SMC element 2, MMS21 homolog; non-structural maintenance of chromosomes element 2 homolog; NSE2; NSMCE2/PVT1 fusion; PVT1/NSMCE2 fusion; zinc finger, MIZ-type containing 7; ZMIZ7
- HostMouse
- IsotypeIgG1
- Protein IDQ96MF7
- Protein NameE3 SUMO-protein ligase NSE2
- Scientific DescriptionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203