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ProductsBack/NSE2 antibody [6A6]
ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83995 NSE2 antibody [6A6].
ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83995 NSE2 antibody [6A6].
ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83995 NSE2 antibody [6A6].

NSE2 antibody [6A6]

GTX83995
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNSMCE2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NSE2 antibody [6A6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6A6
  • Concentration
    0.52 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID286053
  • Target name
    NSMCE2
  • Target description
    NSE2 SUMO ligase component of SMC5/6 complex
  • Target synonyms
    C8orf36, MMS21, NSE2, ZMIZ7, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2, NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase, NSMCE2/PVT1 fusion, PVT1/NSMCE2 fusion, methyl methanesulfonate sensitivity gene 21, non-SMC element 2, MMS21 homolog, non-structural maintenance of chromosomes element 2 homolog, zinc finger, MIZ-type containing 7
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ96MF7
  • Protein Name
    E3 SUMO-protein ligase NSE2
  • Scientific Description
    E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NSMCE2 using anti-NSMCE2 antibody (A06693-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSMCE2 antigen affinity purified polyclonal antibody (Catalog # A06693-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSMCE2 at approximately 34 kDa. The expected band size for NSMCE2 is at 28 kDa.
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