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ICC/IF analysis of A549 cells using GTX87233 NT5C1A antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of A549 cells using GTX87233 NT5C1A antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of A549 cells using GTX87233 NT5C1A antibody. The picture on the right is blocked with the synthesized peptide.

NT5C1A antibody

GTX87233
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNT5C1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    NT5C1A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. ICC/IF: 1:100~1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84618
  • Target name
    NT5C1A
  • Target description
    5'-nucleotidase, cytosolic IA
  • Target synonyms
    CN-I, CN-IA, CN1, CN1A, CNI, cytosolic 5'-nucleotidase 1A, 5'-deoxynucleotidase, AMP-specific 5'-NT, cytosolic 5' nucleotidase, type 1A, cytosolic 5'-nucleotidase IA
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT5C1A using anti-NT5C1A antibody (A12036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C1A antigen affinity purified polyclonal antibody (Catalog # A12036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5C1A at approximately 41 kDa. The expected band size for NT5C1A is at 41 kDa.
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