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NT5C1A / CN1A Antibody (Internal)

LS-C368614
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNT5C1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NT5C1A / CN1A Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF, WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84618
  • Target name
    NT5C1A
  • Target description
    5'-nucleotidase, cytosolic IA
  • Target synonyms
    CN-I, CN-IA, CN1, CN1A, CNI, cytosolic 5'-nucleotidase 1A, 5'-deoxynucleotidase, AMP-specific 5'-NT, cytosolic 5' nucleotidase, type 1A, cytosolic 5'-nucleotidase IA
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT5C1A using anti-NT5C1A antibody (A12036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C1A antigen affinity purified polyclonal antibody (Catalog # A12036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5C1A at approximately 41 kDa. The expected band size for NT5C1A is at 41 kDa.
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