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NUDT6 Antibody (clone OTI10B4, Carrier-free)

LS-C785578
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetNUDT6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUDT6 Antibody (clone OTI10B4, Carrier-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:200 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI10B4
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11162
  • Target name
    NUDT6
  • Target description
    nudix hydrolase 6
  • Target synonyms
    ASFGF2, FGF-AS, FGF2AS, GFG-1, GFG1, nucleoside diphosphate-linked moiety X motif 6, antisense basic fibroblast growth factor, nudix (nucleoside diphosphate linked moiety X)-type motif 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDT6 using anti-NUDT6 antibody (A08301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat H9C2 whole cell lysates, Lane 11: mouse kidney tissue lysates, Lane 12: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT6 antigen affinity purified polyclonal antibody (Catalog # A08301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT6 at approximately 72 kDa. The expected band size for NUDT6 is at 72 kDa.
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