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NUP98 Antibody

LS-C813060
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNUP98
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUP98 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4928
  • Target name
    NUP98
  • Target description
    nucleoporin 98 and 96 precursor
  • Target synonyms
    ADIR2; GLFG-repeat containing nucleoporin; nuclear pore complex protein Nup98; nuclear pore complex protein Nup98-Nup96; nucleoporin 96; nucleoporin 98kD; nucleoporin 98kDa; NUP196; NUP96; NUP98/PHF23 fusion 2 protein; Nup98-96; Nup98-Nup96
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP98 using anti-NUP98 antibody (A01301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP98 antigen affinity purified polyclonal antibody (Catalog # A01301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP98 at approximately 98,105 kDa. The expected band size for NUP98 is at 198 kDa.
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