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NVL Polyclonal Antibody

RD219086A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNVL
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    NVL Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.44 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4931
  • Target name
    NVL
  • Target description
    nuclear VCP like
  • Target synonyms
    NVL2, nuclear valosin-containing protein-like, NVLp
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15381
  • Protein Name
    Nuclear valosin-containing protein-like
  • Scientific Description
    This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. - This is a NVL Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NVL using anti-NVL antibody (A05446-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NVL antigen affinity purified polyclonal antibody (Catalog # A05446-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NVL at approximately 95 kDa. The expected band size for NVL is at 95 kDa.
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