Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_T-44746_20220819_IHC-P_M_22083119_309.webp "O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_T-44746_20220819_IHC-P_R_22083119_191.webp "O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_T-44746_20220916_ICC_IF_22102723_359.webp "O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_44823_20230728_WB_shRNA_watermark_23073119_858.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_44823_20230908_WB_Drosophila_23091319_937.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637222/GTX637222_T-44746_20240126_WB_TPM_watermark_24013018_948.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
O-GlcNAc transferase antibody [HL1641]
GTX637222
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetOGT
Overview
- SupplierGeneTex
- Product NameO-GlcNAc transferase antibody [HL1641]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1641
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8473
- Target nameOGT
- Target descriptionO-linked N-acetylglucosamine (GlcNAc) transferase
- Target synonymsHINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-linked N-acetylglucosamine transferase 110 kDa subunit, UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
- HostRabbit
- IsotypeIgG
- Protein IDO15294
- Protein NameUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
- Scientific DescriptionThis gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
- ReactivityDrosophila, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 Aμg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220805_WB_M_R_22080923_335.webp)
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