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Western blot analysis of extracts of various cell lines using OAS3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using OAS3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using OAS3 Polyclonal Antibody at dilution of 1:3000.

OAS3 Polyclonal Antibody

E-AB-65627
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    OAS3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes an enzyme included in the 2, 5 oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2, 5 oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OAS3 using anti-OAS3 antibody (A05032-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAS3 antigen affinity purified polyclonal antibody (Catalog # A05032-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAS3 at approximately 123KD. The expected band size for OAS3 is at 123KD.
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