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IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml
IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml
IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml

Occludin antibody

GTX85016
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetOCLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Occludin antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Scientific Description
    Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
    Read more
  • Alteration of Gut Microbiota and Inflammatory Cytokine/Chemokine Profiles in 5-Fluorouracil Induced Intestinal Mucositis. Li HL et al., 2017, Front Cell Infect Microbiol
    Read more

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Figure 1. Western blot analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (Catalog # A01246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/OCLN at approximately 65KD. The expected band size for Occludin/OCLN is at 59KD.
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