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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OCLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 53, 55, 32, 24, 9 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OCLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 53, 55, 32, 24, 9 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OCLN antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 53, 55, 32, 24, 9 kDa Observed band size: 60 kDa

OCLN Antibody

CSB-PA016263LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOCLN
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Overview

  • Supplier
    Cusabio
  • Product Name
    OCLN Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Scientific Description
    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (Catalog # A01246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/OCLN at approximately 65KD. The expected band size for Occludin/OCLN is at 59KD.
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