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Western blot analysis of 293T using Occludin Polyclonal Antibody at 1:2000 dilution.
Western blot analysis of 293T using Occludin Polyclonal Antibody at 1:2000 dilution.
Western blot analysis of 293T using Occludin Polyclonal Antibody at 1:2000 dilution.

OCLN Polyclonal Antibody

E-AB-64286
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    OCLN Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Occludin/Ocln using anti-Occludin/Ocln antibody (A01246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/Ocln antigen affinity purified polyclonal antibody (Catalog # A01246-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/Ocln at approximately 59 kDa. The expected band size for Occludin/Ocln is at 59 kDa.
Product group Antibodies
Boster Bio
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ApplicationsWestern Blot, ELISA
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293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Product group Antibodies
References
Bioss
OCLN Polyclonal AntibodyBS-1495R
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ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetOcln
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Product group Antibodies
Biomatik
Occludin (OCLN) Polyclonal AntibodyCAU24528
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