More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/OCLN Polyclonal Antibody

OCLN Polyclonal Antibody

RD70518A
Reddot Biotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetOcln
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    OCLN Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.56 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18260
  • Target name
    Ocln
  • Target description
    occludin
  • Target synonyms
    Ocl, occludin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ61146
  • Protein Name
    Occludin
  • Scientific Description
    Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains,intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms,owing to modifications at the posttranscriptional and posttranslational levels . - This is a OCLN Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of Occludin/Ocln using anti-Occludin/Ocln antibody (A01246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/Ocln antigen affinity purified polyclonal antibody (Catalog # A01246-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/Ocln at approximately 59 kDa. The expected band size for Occludin/Ocln is at 59 kDa.
Product group Antibodies
Boster Bio
Anti-Occludin/Ocln Antibody Picoband(r)A01246-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetOcln
  • SizePrice
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Product group Antibodies
References
Bioss
OCLN Polyclonal AntibodyBS-1495R
ApplicationsFlow Cytometry, Western Blot
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetOcln
  • SizePrice
Product group Antibodies
Biomatik
Occludin (OCLN) Polyclonal AntibodyCAU24528
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetOcln
  • SizePrice