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WB analysis of various sample lysates using GTX33374 OGDH antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33374 OGDH antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33374 OGDH antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

OGDH antibody

GTX33374
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetOGDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    OGDH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4967
  • Target name
    OGDH
  • Target description
    oxoglutarate dehydrogenase
  • Target synonyms
    AKGDH, E1k, E1o, HsOGDH, KGD1, OGDC, OGDH-E1, OGDH2, OGDHD, 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, OGDC-E1, alpha-KGDH-E1, oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide), oxoglutarate decarboxylase, oxoglutarate dehydrogenase (succinyl-transferring), testicular tissue protein Li 131, thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02218
  • Protein Name
    2-oxoglutarate dehydrogenase complex component E1
  • Scientific Description
    This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cardenas C, Lovy A, Silva-Pavez E, et al. Cancer cells with defective oxidative phosphorylation require endoplasmic reticulum-to-mitochondria Ca(2+) transfer for survival. Sci Signal. 2020,13(640). doi: 10.1126/scisignal.aay1212
    Read this paper
  • Green SR, Storey KB. Regulation of the α-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardson's ground squirrel (Urocitellus richardsonii). Biochim Biophys Acta Proteins Proteom. 2020,1868(9):140448. doi: 10.1016/j.bbapap.2020.140448
    Read this paper
  • Leandro J, Dodatko T, Aten J, et al. DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. Hum Mol Genet. 2020,29(7):1168-1179. doi: 10.1093/hmg/ddaa037
    Read this paper

Datasheet

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Figure 1. Western blot analysis of OGDH using anti-OGDH antibody (A05301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGDH antigen affinity purified polyclonal antibody (Catalog # A05301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGDH at approximately 110 kDa. The expected band size for OGDH is at 116 kDa.
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