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OLFM4 Rabbit pAb, HRP conjugated

ORB110355
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    OLFM4 antibody (HRP)
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB=1:500-2000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    HRP
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    OLFM4 Rabbit pAb, HRP conjugated
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human U2OS whole cell lysates Lane 3: human U-87MG whole cell lysates Lane 4: human HL-60 whole cell lysates Lane 5: human K562 whole cell lysates Lane 6: human THP-1 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.
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