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WB analysis of human amygdala lysate using GTX89414 OMG antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human amygdala lysate using GTX89414 OMG antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human amygdala lysate using GTX89414 OMG antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

OMG antibody, Internal

GTX89414
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetOMG
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Overview

  • Supplier
    GeneTex
  • Product Name
    OMG antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4974
  • Target name
    OMG
  • Target description
    oligodendrocyte myelin glycoprotein
  • Target synonyms
    oligodendrocyte-myelin glycoprotein; OMGP
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP23515
  • Protein Name
    Oligodendrocyte-myelin glycoprotein
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OMG using anti-OMG antibody (PB9730). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMG antigen affinity purified polyclonal antibody (Catalog # PB9730) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMG at approximately 50KD. The expected band size for OMG is at 50KD.
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