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ProductsBack/Oncostatin M (OSM) Mouse Monoclonal Antibody [Clone ID: OTI2B6]
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-OSMmouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-OSMmouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-OSMmouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Oncostatin M (OSM) Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CF500195
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOSM
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Oncostatin M (OSM) Mouse Monoclonal Antibody [Clone ID: OTI2B6]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2B6
  • Gene ID5008
  • Target name
    OSM
  • Target description
    oncostatin M
  • Target synonyms
    oncostatin-M
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13725
  • Protein Name
    Oncostatin-M
  • Scientific Description
    Carrier-free (BSA/glycerol-free) OSM mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA09694A0Rb diluted at 1:90 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HUT Whole Cell Lysate, Lane 3: JURKAT Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate, Lane 5: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # PB9732) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 40 kDa. The expected band size for Oncostatin M is at 28 kDa.
Product group Antibodies
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