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OPA1 Antibody

HY-P80255
MedChem Express
TargetOPA1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    OPA1 Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4976
  • Target name
    OPA1
  • Target description
    OPA1 mitochondrial dynamin like GTPase
  • Target synonyms
    BERHS; dynamin-like 120 kDa protein, mitochondrial; dynamin-like guanosine triphosphatase; largeG; MGM1; mitochondrial dynamin-like GTPase; MTDPS14; NPG; NTG; optic atrophy 1 (autosomal dominant); optic atrophy protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    OPA1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 112 kDa, targeting to OPA1. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA016340HA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of OPA1 using anti-OPA1 antibody (A00508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse eye tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # A00508-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100 kDa. The expected band size for OPA1 is at 112,80-100 kDa.
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