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WB analysis of Jurkat cell lysate using GTX85443 ORAI2 antibody. Working concentration : (A) 1, (B) 2, and (C) 4 microg/ml
WB analysis of Jurkat cell lysate using GTX85443 ORAI2 antibody. Working concentration : (A) 1, (B) 2, and (C) 4 microg/ml
WB analysis of Jurkat cell lysate using GTX85443 ORAI2 antibody. Working concentration : (A) 1, (B) 2, and (C) 4 microg/ml

ORAI2 antibody

GTX85443
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetORAI2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ORAI2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 4 microg/mL. ICC/IF: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80228
  • Target name
    ORAI2
  • Target description
    ORAI calcium release-activated calcium modulator 2
  • Target synonyms
    C7orf19; CAP-binding protein complex interacting protein 2; CBCIP2; H_NH0514P08.8; MEM142B; protein orai-2; putative protein ORAI2-2; TMEM142B; transmembrane protein 142B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SN7
  • Protein Name
    Protein orai-2
  • Scientific Description
    Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Orai2 using anti-Orai2 antibody (A07411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Orai2 antigen affinity purified polyclonal antibody (Catalog # A07411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Orai2 at approximately 39KD. The expected band size for Orai2 is at 39KD.
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